SopB protein-mediated silencing of genes linked to the sopC locus of Escherichia coli F plasmid.

Localization of F plasmid SopB protein to positions near the poles of Escherichia coli cells.

The subcellular localization of the SopB protein, which is encoded by the Escherichia coli F plasmid and is involved in the partition of the single-copy plasmid, was directly visualized through the expression of the protein fused to the jellyfish green fluorescent protein (GFP). The fusion protein, but not GFP itself, was found to localize to positions close but not at the poles of exponentiall...

multiplex pcr study of plasmid-mediated ampc beta-lactamase genes in clinical isolates of escherichia coli

background :   ampc β-lactamases are important cephalosporinases chromosomally encoded in many of enterobacteriaceae and a few other organisms where they mediate resistance to cephalothin, cefazolin, cefoxitin and penicillins. the six different families of plasmid-mediated ampc β-lactamases have been described, but no phenotypic test can discriminate among them. ampc multiplex pcr has been succ...

Insight into F plasmid DNA segregation revealed by structures of SopB and SopB–DNA complexes

Accurate DNA segregation is essential for genome transmission. Segregation of the prototypical F plasmid requires the centromere-binding protein SopB, the NTPase SopA and the sopC centromere. SopB displays an intriguing range of DNA-binding properties essential for partition; it binds sopC to form a partition complex, which recruits SopA, and it also coats DNA to prevent non-specific SopA-DNA i...

Centromere binding specificity in assembly of the F plasmid partition complex

The segregation of plasmid F of Escherichia coli is highly reliable. The Sop partition locus, responsible for this stable maintenance, is composed of two genes, sopA and sopB and a centromere, sopC, consisting of 12 direct repeats of 43 bp. Each repeat carries a 16-bp inverted repeat motif to which SopB binds to form a nucleoprotein assembly called the partition complex. A database search for s...

PCR Detection of Plasmid Mediated TEM, SHV and AmpC β-Lactamases in Community and Nosocomial Urinary Isolates of Escherichia coli